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Lab Test Details

Influenza Type A and B, Qualitative Real-Time RT-PCR with Reflex

Updated

06/27/2019

Search Terms Flu A and B PCR
How to Order On the Virology/Immunology Test Request Form (OHA0042), in the “Other” section, check the box for “Influenza RT-PCR Screen.”

If the specimen is associated with an identified outbreak, include the Outbreak Number in the Outbreak Number box on the test request form.
Availability All clients with approval from the Acute and Communicable Disease Prevention Program (ACDP)
Performed Once per week or more, depending on demand
Time to Reporting Within 7 working days after receipt of specimen
CPT Code 87798 x 2. If Influenza A is detected, additionally 87798 x 4 (H1, H3, H1N1 (2009) pdm InfA, and HIN1 (2009) pdm H1)


Specimen Collection Details

Collection Collect specimen(s) using Dacron polyester tips or flocked swabs on a plastic shaft (wooden shaft swabs are not acceptable). Nasopharyngeal swabs are preferred. Ideally, collect specimens within 3 days of illness onset and not later than 7 days after illness onset. Insert swabs into viral transport media. Do not submit a dry swab for testing.

Instructions for collecting nasopharyngeal specimens are available from Copan.  

Acceptable specimen sources include: Nasopharyngeal swabs (preferred), nasal swabs, throat swabs, combination nasopharyngeal swabs (2 swabs in one vial), nasal aspirates, nasal washes, bronchoalveolar lavages, bronchial washes, tracheal aspirates, sputum, lung tissue, or cell culture isolates.
Optimal Volume 2-3 mL
Minimum Volume 1 mL
Handling

Store specimens at refrigerated temperatures (2-8°C) pending transport.

If specimen transport cannot occur within 2-3 days of specimen collection, freeze the specimen(s), preferably at -70° C, and ship on dry ice as soon as possible.
Transport Package specimens with fully frozen ice packs to maintain refrigerated temperatures during transport. Transport for receipt at the OSPHL within 3 days of specimen collection.

If specimen is frozen, package specimens with dry ice to maintain frozen temperatures during transport.
Rejection Criteria Swabs with calcium alginate, cotton tips, or wooden shafts; specimens that are collected more than 14 days after illness onset.

Specimens are subject to the requirements of the OSPHL Specimen Submission Policy and Criteria, available at: http://bit.ly/SpecimenCriteria.
Comments This test will identify influenza A H3, seasonal H1, 2009 H1 subtypes, and H3N2 variant, in addition to influenza B. Highly pathogenic avian influenza testing (e.g., H5 and H7) available if approved

Method Details

Methodology

Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)

Performance Specifications
Performance specifications will be posted at a later date. Please contact the OSPHL for performance specifications in the interim. 

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