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Lab Test Details

Vibrio spp. Identification

Updated8/14/2025
Search Terms V. alginolyticus; V. cholerae; V. cidicii; V. cincinatiensis; V. fluvialis; V. furnissii; V. harveyi; V. metoecus; V. metschnikovii; V. mimicus; V. navarrensis; V. parahaemolyticus; and V. vulnificus; Grimontia hollisae
How to OrderIf submitting an isolate, on the General Microbiology Test Request Form (OHA0060), in the "Enterics" section, check the "VIBID" box in the "Vibrio spp." row.

If submitting original material, follow the instructions for Enteric Pathogen Culture.

If previous testing was conducted using a culture-independent diagnostic test (CIDT), in the "Specimen/Isolate Information" section, for "Was an original test performed on a CIDT platform?" select "Yes" and attach your laboratory's result reports.
AvailabilityAll clients
PerformedDaily on regular business days
Time to ReportingFor isolates or original material submitted, within 7 working days after receipt of specimen

Preliminary report will be sent upon isolate confirmation followed by a final report for species identification.
Public Health DataDe-identified data from sequencing foodborne pathogenic bacteria are submitted to the national repository bank, NCBI, and also shared with the Centers for Disease Control and Prevention, PulseNet, as part of genomic surveillance and epidemiological data sharing.
CPT Code

Specimen Collection Details

CollectionIf submitting an isolate (preferred), submit a pure culture of isolate on an agar slant or non-selective plate media.

If submitting original material for culture, please follow the Specimen Collection Details for the Enteric Pathogen Culture.
Optimal VolumeN/A
Minimum VolumeN/A
HandlingSeal plate, place in biohazard transport bag. Store plate at room temperatures (20-25°C) pending transport.
TransportPackage plate to maintain room temperatures during transport.

Do not collect or transport for receipt at the OSPHL on a state holiday. Sample integrity may be compromised.
Rejection CriteriaSpecimens are subject to the requirements of the OSPHL Specimen Submission Policy (pdf).
Comments Vibrio cholerae strains will be evaluated for confirmation of serogroups O1 and O139.

Original material submitted for testing is retained for 5 calendar days after receipt at the laboratory.

For additional information or assistance, please contact the OSPHL at 503-693-4100.

Method Details

MethodologyCulture

Whole genome sequencing identification by PulseNet 2.0 beginning 8/20/2025
Performance
Specifications
Culture: N/A

Whole Genome Sequencing: 
Accuracy: 100%
Sensitivity: 100%
Specificity: 100%



Change Log

8/14/2025
Resume WGS identification using PulseNet 2.0: Time to Reporting, Methodology, and Performance Specifications updated. 
2/19/2025
Updated How to Order; Link in Public Health Data; Rejection Criteria; Comments
9/12/2024Temporary changes to Time to Reporting, Methodology, and Performance Specifications; Edited Comments
8/31/2023Implementation of species identification by ANI: Updated Search Terms; Time to Reporting; Methodology; Performance Specifications; Edited How to Order and Comments
5/2/2023Added Public Health Data section