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Test Menu Details


E. coli O157 Identification

Updated

2/19/2025

Search Terms
How to Order

On the General Microbiology Test Request Form (OHA0060), in the Enterics section, choose the "O157 ID" box in the “Shiga toxin producing E. coli” row.

If submitting original material, follow the instructions for Enteric Pathogen Culture. 

If previous testing was conducted using a culture-independent diagnostic test (CIDT), in the "Specimen/Isolate Information" section, for "Was an original test performed on a CIDT platform?" select "Yes" and attach your laboratory's result reports.

Availability

All clients

Performed

Daily on regular business days

Time to Reporting

For isolates or original material submitted: Within 7 working days after receipt of specimen

Preliminary report will be sent upon isolate confirmation, followed by a final report for identification.

Public Health Data
De-identified data from sequencing foodborne pathogenic bacteria are submitted to the national repository bank, NCBI, and also shared with the Centers for Disease Control and Prevention, PulseNet, as part of genomic surveillance and epidemiological data sharing.
CPT Code


Specimen Collection Details

Collection

Submit pure isolate on a slant or on Agar plate supporting growth.

If submitting original material for culture, please follow the Specimen Collection Details for Enteric Pathogen Culture.

Optimal Volume

N/A

Minimum Volume

N/A

Handling

Tightly seal slant or plate with paraffin wrap. Store specimens at room temperatures (20-25°C) pending transport.

Transport
Do not collect or transport for receipt at the OSPHL on a state holiday. Sample integrity may be compromised. 

Package specimens to maintain room temperatures during transport.

Rejection CriteriaSpecimens are subject to the requirements of the OSPHL Specimen Submission Policy (pdf).
CommentsSample integrity may be compromised if received at the OSPHL on a state holiday. Avoid sending samples for receipt at the laboratory on a state holiday.

Original material submitted for testing is retained for 5 calendar days after receipt at the laboratory.

For additional information or assistance, please contact the OSPHL at 503-693-4100.

Method Details

Methodology

Multiplex RT-PCR for Shiga Toxin detection, biochemical and serological identification

Performance
Specifications

Biochemical and serological identification - N/A

Multiplex RT-PCR for Shiga Toxin detection

  • Accuracy
    • 100% for isolates
    • 98.8% for broths
  • Sensitivity
    • 100% for isolates
    • 98.4% for broths
  • Specificity
    • 100% for isolates
    • 93.3% for broths
  • Limit of Detection (LOD) - Target genes
    • stx1 = 10 genome copies
    • stx2 = 10 genome copies
    • eae = 10 genome copies


Change Log

2/19/2025
Updated How to Order; Link in Public Health Data; Collection; Rejection Criteria
4/24/2023
Added Public Health Data section